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goat anti mpo polyclonal antibody  (R&D Systems)
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Neutrophils were stained <t>with</t> <t>anti–MPO-FITC,</t> SYTOX Orange, Hoechst, and Live/Dead stain and visualized for fluorescence and bright-field (BF). ( A and B ) Gating strategy ( A ) of neutrophils subpopulations with representative images shown ( B ). ( C – G ) Frequency of NETs and NET precursors (Type III and II) ( C ), NET fragments (Type IV) ( D ), dead cells with condensed nuclei (Type V) ( E ), dead cells with decondensed nuclei (Type VI) ( F ), and live cells (Type I) ( G ). ( H ) Frequency of Hoechst + cells. ( I ) Circularity scores across groups. Experiments were performed in 8 experiments and combined, n = 8. Box-and-whisker plots show median, all points, and 25–75th percentiles ( C – I ). Data were analyzed by 1-way ANOVA with Holm-Šídák’s multiple-comparisons test ( C – I ). * P < 0.05. Sia, sialic acid.
Goat Anti Mpo Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Neutrophils were stained <t>with</t> <t>anti–MPO-FITC,</t> SYTOX Orange, Hoechst, and Live/Dead stain and visualized for fluorescence and bright-field (BF). ( A and B ) Gating strategy ( A ) of neutrophils subpopulations with representative images shown ( B ). ( C – G ) Frequency of NETs and NET precursors (Type III and II) ( C ), NET fragments (Type IV) ( D ), dead cells with condensed nuclei (Type V) ( E ), dead cells with decondensed nuclei (Type VI) ( F ), and live cells (Type I) ( G ). ( H ) Frequency of Hoechst + cells. ( I ) Circularity scores across groups. Experiments were performed in 8 experiments and combined, n = 8. Box-and-whisker plots show median, all points, and 25–75th percentiles ( C – I ). Data were analyzed by 1-way ANOVA with Holm-Šídák’s multiple-comparisons test ( C – I ). * P < 0.05. Sia, sialic acid.
Goat Polyclonal Antibody R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Neutrophils were stained <t>with</t> <t>anti–MPO-FITC,</t> SYTOX Orange, Hoechst, and Live/Dead stain and visualized for fluorescence and bright-field (BF). ( A and B ) Gating strategy ( A ) of neutrophils subpopulations with representative images shown ( B ). ( C – G ) Frequency of NETs and NET precursors (Type III and II) ( C ), NET fragments (Type IV) ( D ), dead cells with condensed nuclei (Type V) ( E ), dead cells with decondensed nuclei (Type VI) ( F ), and live cells (Type I) ( G ). ( H ) Frequency of Hoechst + cells. ( I ) Circularity scores across groups. Experiments were performed in 8 experiments and combined, n = 8. Box-and-whisker plots show median, all points, and 25–75th percentiles ( C – I ). Data were analyzed by 1-way ANOVA with Holm-Šídák’s multiple-comparisons test ( C – I ). * P < 0.05. Sia, sialic acid.
Goat 563 Anti Mpo Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat polyclonal anti mpo  (R&D Systems)
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Goat Polyclonal Anti Mpo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti human mpo polyclonal antibody  (R&D Systems)
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Neutrophils were stained with anti–MPO-FITC, SYTOX Orange, Hoechst, and Live/Dead stain and visualized for fluorescence and bright-field (BF). ( A and B ) Gating strategy ( A ) of neutrophils subpopulations with representative images shown ( B ). ( C – G ) Frequency of NETs and NET precursors (Type III and II) ( C ), NET fragments (Type IV) ( D ), dead cells with condensed nuclei (Type V) ( E ), dead cells with decondensed nuclei (Type VI) ( F ), and live cells (Type I) ( G ). ( H ) Frequency of Hoechst + cells. ( I ) Circularity scores across groups. Experiments were performed in 8 experiments and combined, n = 8. Box-and-whisker plots show median, all points, and 25–75th percentiles ( C – I ). Data were analyzed by 1-way ANOVA with Holm-Šídák’s multiple-comparisons test ( C – I ). * P < 0.05. Sia, sialic acid.

Journal: JCI Insight

Article Title: Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection

doi: 10.1172/jci.insight.180024

Figure Lengend Snippet: Neutrophils were stained with anti–MPO-FITC, SYTOX Orange, Hoechst, and Live/Dead stain and visualized for fluorescence and bright-field (BF). ( A and B ) Gating strategy ( A ) of neutrophils subpopulations with representative images shown ( B ). ( C – G ) Frequency of NETs and NET precursors (Type III and II) ( C ), NET fragments (Type IV) ( D ), dead cells with condensed nuclei (Type V) ( E ), dead cells with decondensed nuclei (Type VI) ( F ), and live cells (Type I) ( G ). ( H ) Frequency of Hoechst + cells. ( I ) Circularity scores across groups. Experiments were performed in 8 experiments and combined, n = 8. Box-and-whisker plots show median, all points, and 25–75th percentiles ( C – I ). Data were analyzed by 1-way ANOVA with Holm-Šídák’s multiple-comparisons test ( C – I ). * P < 0.05. Sia, sialic acid.

Article Snippet: Cells were incubated with primary antibodies: sheep anti-THP polyclonal antibody (1:40, R&D Systems, AF5175) conjugated using the FITC Conjugation Kit Lightning-Link Abcam, ab102884), goat anti-MPO polyclonal antibody (1:200, R&D Systems, AF3667), and rabbit anti–Histone H3 antibody (1:100, Abcam, ab5103) in PBST+1% BSA.

Techniques: Staining, Fluorescence, Whisker Assay

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Granulocyte colony stimulating factor promotes scarless tissue regeneration

doi: 10.1016/j.celrep.2024.114742

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following primary antibodies were used: rabbit monoclonal anti-Arginase-1 (93668, Cell Signaling Technology), rabbit monoclonal anti-Ki-67 (9129, Cell Signaling), rabbit polyclonal anti-CD80 (8679, ProSci), rabbit polyclonal anti-Histone H3 (ab5103, Abcam), goat polyclonal anti-MPO (AF3667, R&D Systems), rabbit monoclonal anti-MRC1 (24595, Cell Signaling), rat monoclonal anti-F4/80 (ab6640, Abcam), rabbit polyclonal anti-Neutrophil Elastase (ab68672, Accam), mouse monoclonal anti-cytokeratin 14 (ab7800, Abscam), rabbit monoclonal anti keratin 6 (SAB5500131, Sigma), rabbit monoclonal anti-Phospho-Histone H3 (53348, Cell Signaling), rabbit monoclonal anti-CD31 (77699, Cell Signaling), rabbit monoclonal anti-F4/80 (70076, Cell Signaling), rabbit polyclonal anti-COX2 (ab15191, Abcam), rabbit anti-iNOS (2982, Cell Signaling), rabbit polyclonal anti-GCSFR (bs-2574R, Bioss) rabbit monoclonal anti-CD163 (68922, Cell Signaling) and rat anti-Ly6G (551459, BD Pharmingen).

Techniques: Recombinant, Conjugation Assay, Enzyme-linked Immunosorbent Assay, Software